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  • Title: [Retinal sensitized photochemiluminescence of trypsin solutions and the effect of 3-hydroxypyridines].
    Author: Shiriaev VM, Fedorovich IB, Sapezhinskiĭ II, Khristoforov VL, Kuz'min VI.
    Journal: Biofizika; 1980; 25(3):439-45. PubMed ID: 7397260.
    Abstract:
    A study of retinal sensibilized photochemiluminescence of trypsin is carried out. A kinetic scheme of processes responsible for the luminescence appearance according to which chemiluminescence depends on protein groups reaction with singlet oxygen is suggested. The values of a number of kinetic parameters of these processes are determined. The effect of 3-oxypyridine on retinal sensibilized photochemiluminescence is studied. The 3-oxypyridine addition to protein solution with retinal before radiation considerably decreases the intensity and light sum of the luminescence. 2-Oxypyridine photoprotective action is due to their reaction with primary and peroxide radicals of protein. The values of rate constants of interaction of protein radicals with 2,6-dimethyl-3-oxypyridine and 2-ethyl-6-methyl-3-oxypyridine are determined.
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