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  • Title: Cystic fibrosis serum alpha-l-fucosidase: confirmation of normal activity levels and normal kinetic and isoelectric focusing properties.
    Author: Alhadeff JA, Watkins P.
    Journal: Clin Chim Acta; 1980 Jul 17; 105(1):131-5. PubMed ID: 7398081.
    Abstract:
    Comparable alpha-L-fucosidase activity was found for normal and cystic fibrosis sera, 7.8 +/- 3.3 and 9.8 +/- 4.7 nmol/min/ml, respectively. Isoelectric focusing of normal and cystic fibrosis sera alpha-L-fucosidase revealed similar isoelectric profiles with most activity between isoelectric points of 4.7--5.4. Kinetic analysis of normal and cystic fibrosis sera alpha-L-fucosidase revealed identical apparent Michaelis constants for the 4-methylumbelliferyl substrate (51 +/- 6 mumol/l and 50 +/- 8 mumol/l, respectively), similar pH optima curves (both have broad optima between pH 4.8 and 6.5) and identical thermostability curves at three different preincubation temperatures (37 degrees, 45 degrees and 55 degrees C).
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