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  • Title: Cleavage of pre-mRNA sequences by ribonucleases bound to nuclear RNP particles of rat liver.
    Author: Molnár J, Bajszár G, Marczinovits I, Szabó G.
    Journal: Mol Biol Rep; 1978 Oct 16; 4(3):157-61. PubMed ID: 739982.
    Abstract:
    The 30S nuclear RNP particles from rat liver have been shown to split the double-stranded- (ds) and single-stranded (ss) sequences of nuclear pre-mRNA. Experiments performed in vitro have demonstrated that 1) a 5'-exonuclease and an endonuclease specific for double-stranded pre-mRNA sequences exist in the 30S pre-mRNP particles; 2) in dsRNA monophosphorylated 5'-termini arose in the course of incubation with 30S RNP and most of the products remained double-stranded. The analysis of terminal pNp nucleotides revealed a relatively high ratio of pPyp in the cleaved dsRNA, whereas the nucleosides in 5'-terminal pNp of ssRNA showed nearly random distribution. Our results provide a possible explanation for the appearance of pNp termini during the processing of nuclear pre-mRNA of mammalian cells.
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