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  • Title: Quantitation of oestrogen receptors: use of solid-phase antisteroid antibodies to quantify binding sites and determination of dissociation constant.
    Author: Raam S, Cohen JL.
    Journal: J Clin Pathol; 1980 Apr; 33(4):377-9. PubMed ID: 7400336.
    Abstract:
    The dextran-coated charcoal assay method of McGuire and DeLaGarza is a well-known method for quantitation of oestrogen receptors in breast tumour tissues. Castañeda and Liao reported that the solid-phase antisteroid antibodies are superior to dextran-coated charcoal for quantitating the receptor bound steroids. In this report we compare the results obtained using both methods to quantitate oestrogen receptors and determine the affinity for steroid binding in seven human breast tumours and the uteri of rat and calf. This study compared the results obtained for quantifying estrogen receptors in breast and uterine tissues using either the dextran-coated charcoal assay or a solid-phase antisteroid antibody assay system. 7 human breast tumors and the uteri of rat and calf were used for the comparisons. In addition to quantifying estrogen receptors, the affinity for steroid binding was also determined. Good agreement was found between the values for estimated sites per milligram of cytosol protein for all the samples assayed by both methods. Dissociation constants were all found to be in the same range as those described by other investigators for estrogen receptors. This agreement was true for both human and animal tissues. Even tumor cytosols with no specific binding showed no significant differences between the 2 methods in the ability to estimate nonspecific binding components in the presence of excess diethylstilbestrol.
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