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  • Title: [L line cell differentiation. Histochemical and electron microscopic studies].
    Author: Shuklinov VA, Livshits VL, Shumilina VV, Tregubova NA, Isakova LM.
    Journal: Tsitologiia; 1980 Jul; 22(7):804-9. PubMed ID: 7404659.
    Abstract:
    In a medium supplemented with 60 per cent large cattle serum, part of the cell population of L-transformed culture was seen to be differentiated into mature lipocytes. The formation of structures similar to normal fat tissue took place in the same conditions, provided a reduced quantity of the seeded cells was used. The cell differentiation was accompanied with a decrease in acid phosphatase and non-specific esterase activities. The ultrastructural study has demonstrated a suitable preservation of cytoplasmic structures and a domination of the rough cytoplasmic reticulum compared to the control cell cultures where free ribosomes and polysomes predominated.
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