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  • Title: Effect of p-chloromercurybenzoate on the MDH-activity of cytosol and rat liver submitochondrial fractions in different buffers.
    Author: Kirkova M, Russanov E.
    Journal: Acta Physiol Pharmacol Bulg; 1980; 6(1):53-9. PubMed ID: 7405594.
    Abstract:
    The effect of p-chloromercurybenzoate (p-CMB) on the MDH-activity of cytosol and submitochondrial fractions (matrix and inner membranes) of rat liver is studied in different buffer systems with identical pH. It is found that p-CMB has two effects on the MDH-activity, depending on the subcellular localization of the enzyme and on the type of buffer used: inhibitory (for the enzyme of the three fractions in HEPES-NaOH and potassium-phosphate buffers) and activating (for the enzyme of the submitochondrial fractions in Tris-HCI buffer). The effect of the SH-reagent on the formation and stability of enzyme-substrate complexes in the different buffers is also studied, as well as the temperature dependence of the p-CMB action on the MDH-activity of the preparations. It is found that the association of m-MDH with the inner mitochondrial membranes does not change its kinetic behaviour. The probable different conformation of the mitochondrial enzyme in the different buffer systems is discussed.
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