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  • Title: Specific techniques for the identification of O-acylated sialic acids in colonic mucins.
    Author: Culling CF, Reid PE.
    Journal: J Microsc; 1980 Aug; 119(3):415-25. PubMed ID: 7411601.
    Abstract:
    The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C4 and side chain O-acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of histopathology is discussed.
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