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  • Title: Purification of low molecular weight metal-binding proteins by preparative polyacrylamide gel electrophoresis: properties of electrophoretically purified rat liver (Cd, Zn) - metallothioneins.
    Author: Zelazowski AJ, Szymańska JA, Witas HW.
    Journal: Prep Biochem; 1980; 10(4):495-505. PubMed ID: 7413609.
    Abstract:
    A method is proposed for purification of metallothionein by preparative polyacrylamide gel electrophoresis. The method enables purification of 100-700 mg of a preparation containing (Cd, Zn) - metallothionein yielding preparations of considerably higher purity as compared with those obtained by ion-exchange chromatography.
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