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  • Title: The simultaneous determination of theophylline, theobromine and caffeine in plasma by high performance liquid chromatography.
    Author: Foenander T, Birkett DJ, Miners JO, Wing LM.
    Journal: Clin Biochem; 1980 Jun; 13(3):132-4. PubMed ID: 7418197.
    Abstract:
    A high performance liquid chromatographic procedure for the stimultaneous micro-scale determination of theophylline, theobromine and caffeine in plasma is described. After a single dichloromethane extraction of 0.5--0.2 ml of acidified plasma, the evaporated residue is chromatographed on a reverse-phase gC-18) column. With a mobile phase of acetate buffer (pH 4)--acetonitrile (88:12) at a flow-rate of 2.0 ml/min., the three methylxanthines are separated within six minutes. Detection at xanthines are separated within six minutes. Detection at 276-280 nm enables quantitation of 0.1 mg/1 of drug in a 0.1 ml sample. The method is reproducible, correlates well with EMIT for plasma theophylline, and is applicable to the routine monitoring of both paediatric and adult patients as well as to metabolic studies.
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