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  • Title: Identification and quantification of alpha-amino adipic acid in bovine dentine phosphoprotein.
    Author: Hiraoka BY, Fukasawa K, Fukasawa KM, Harada M.
    Journal: J Biochem; 1980 Aug; 88(2):373-7. PubMed ID: 7419498.
    Abstract:
    A unique phosphoprotein, which contains an uncommon amino acid, alpha-amino adipic acid (alpha-AAA), was isolated from unerupted bovine teeth by extensive EDTA demineralization. The alpha-AAA in the protein hydrolysates was identified based on the elution time on an amino acid analyzer using the sodium and lithium citrate buffer in a dual column system, and its identity was confirmed by comparisons of its DNS derivative with that of authentic alpha-AAA. This result suggests that the lysine residues in the phosphoprotein may be deaminated by an amine oxidase into allysine and further oxidized to alpha-AAA.
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