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  • Title: Analysis of isoxazolyl penicillins and their metabolites in body fluids by high-performance liquid chromatography.
    Author: Thijssen HH.
    Journal: J Chromatogr; 1980 Sep 12; 183(3):339-45. PubMed ID: 7419651.
    Abstract:
    A high-performance liquid chromatographic assay method to quantitate the isoxazolyl penicillins, their active metabolites, and their penicilloic acids in serum or urine is described. Separation and analysis is performed using reversed-phase chromatography. Urine samples, after the appropriate dilution, can be assayed directly. Serum samples (0.1 ml) are either extracted with methylene chloride or treated with perchloric acid--methanol. Serum levels as low as 0.4 microgram/ml (extraction procedure) can be assayed accurately.
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