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  • Title: Evidence for intracistronic complementation of the product of the influenza virus gene Ptra (P3 of fowl plague virus).
    Author: Heller E, Scholtissek C.
    Journal: J Gen Virol; 1980 Jul; 49(1):133-9. PubMed ID: 7420060.
    Abstract:
    The ts lesion of the fowl plague virus (FPV) mutants ts 18 and ts 236 has been located in RNA segment 2 (Ptra gene, corresponding to P3). After double-infection with these mutants and ts 90 or ts 93, which also carry a ts lesion in segment 2, plaques were formed at the non-permissive temperature (40 degrees C). These plaques cannot be passaged at 40 degrees C and exhibit a morphology which differs from those formed by the wild-type virus. The yield of infectious particles after double-infection shows a non-linear correlation between the plaque number and dilution, indicating that at least two particles are needed for infection of a cell. All experimental evidence points to an intracistronic complementation within the P3 protein.
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