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  • Title: Effect of preincubation in the cold on poly(U)-dependent polyphenylalanine synthesis in a cell-free rat liver system.
    Author: Noll F, Schwedersky G, Bielka H.
    Journal: Acta Biol Med Ger; 1980; 39(1):41-6. PubMed ID: 7424335.
    Abstract:
    Preincubation of a cell-free poly(Phe) synthesis system in the cold results in a manifold stimulation of the polyphenylalanine synthesis. This stimulatory effect can be observed only if ribosomal subunits, poly(U), Mg++ and cytosolic fraction are present in the reaction mixture during cold pretreatment. This effect is abolished if the samples are preincubated at 37 degrees C before the cold treatment probably because of an inactivation of protein factors present in the cytosolic fraction.
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