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  • Title: [Demonstration of antibody and nonspecific immunoglobulin formation in a single cell].
    Author: Sidorova EV, Beliaeva EL.
    Journal: Biull Eksp Biol Med; 1980 Sep; 90(9):336-9. PubMed ID: 7426738.
    Abstract:
    Identification of the products secreted by the single cell is described. The method was used for simultaneous detection of antibodies and nonspecific immunoglobulins (nIg) secreted by the cells of mice immunized with sheep red blood cells (SRBC). The spleen cells were applied to the millipore and cultivated on agarose mixed with native SRBC or with SRBC sensitized with antibodies to mouse Ig. Antibody- and Ig-forming cells (AFC and IFC) were located by the indirect or reverse plaque technique. For nIg-forming cell (nIFC) detection the immobilized antigen (SRBC) was placed between the millipore with the spleen cells and agarose with sensitized SRBC. Comparison of the localization of the plaques on the native SRBC with that on the sensitized SRBC made it possible to ascertain whether the cell produces only antibodies or both antibodies and nIg. It was shown that during the secondary response to SRBC the overwhelming majority of AFC do not produce nIg.
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