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  • Title: A plaque assay for malignant catarrhal fever virus and virus neutralizing activity.
    Author: Hazlett DT.
    Journal: Can Vet J; 1980 May; 21(5):162-4. PubMed ID: 7427840.
    Abstract:
    A cell-free strain of malignant catarrhal fever virus which produced a readily recognizable cytopathic effect was obtained by serial passage of the virus in a rabbit kidney cell line. Plaque assay of the virus was more rapid and gave higher titres 11 days postinoculation than tube titration, but the latter advantage decreased with a longer incubation period. Plaques were clear with sharp edges and measured 0.5 to 2 mm in diameter after 15 days. A plaque neutralization test was developed and successfully employed for the titration of malignant catarrhal fever virus neutralizing activity in the sera and nasal secretions of blue wildebeest.
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