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  • Title: Neuron-specific enolase levels in primary cultures of neurons.
    Author: Schengrund CL, Marangos PJ.
    Journal: J Neurosci Res; 1980; 5(4):305-11. PubMed ID: 7431433.
    Abstract:
    Primary neuronal cell cultures, derived by centrifugal elutriation of cells dissociated from embryonic rat cerebra, have been analyzed for their content of neuron-specific enolase (NSE) and non-neuronal enolase (NNE). Both immunocytochemical staining and radioimmunoassay establish that NSE is present in these cells. Furthermore, the observed increase in NSE and decrease in the NNE/NSE ratio with time in culture parallels that observed in vivo. It has recently been shown that during in vivo neurogenesis and maturation a "switch over" occurs from NNE to NSE which correlates with neuronal differentiation. The presence of a neuron-specific protein in these primary neuronal cultures and the changes observed during growth further support their use as a model system for the study of nervous tissue differentiation.
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