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  • Title: A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain.
    Author: Booth RF, Clark JB.
    Journal: Biochem J; 1978 Nov 15; 176(2):365-70. PubMed ID: 743245.
    Abstract:
    A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with 'free' mitochondria as judged by marker-enzyme assays and electron microscopy.
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