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  • Title: Upon the observation of superbeads in chromatin.
    Author: Muyldermans S, Lasters I, Wyns L, Hamers R.
    Journal: Nucleic Acids Res; 1980 May 24; 8(10):2165-72. PubMed ID: 7433089.
    Abstract:
    There exist some indications that nucleases recognize "superbeads" in chromatin. We show that a chromatin extract of rat liver which contains so-called "superbead"-peaks can be separated in a Mg++ soluble and a Mg++ insoluble fraction. The Mg++ insoluble fraction contains the full complement of histones and the expected DNA fragments, but has lost the characteristic peaks in sucrosegradient profiles. These discrete peaks are found in the Mg2+ soluble fraction of the chromatin extract. We give evidence that these peaks are RNP particles on the basis of their protein- and nucleic acid contents.
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