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  • Title: [Levanase from Fusarium solani-68].
    Author: Elisashvili VI, Loitsianskaia MS, Baranova TI, Bangura A.
    Journal: Prikl Biokhim Mikrobiol; 1980; 16(3):342-6. PubMed ID: 7433428.
    Abstract:
    Extracellular levanase was isolated from the culture of Fusarium solani-68 by ammonium sulfate precipitation at 0.8 saturation and purified in part by Sephadex G-150 gel chromatography. Levanase showed maximum activity at pH = 6.0 and t = 45 degrees C. The levanase reaction (hydrolysis of high molecular levane with a molecular weight of 1--5 min) reached a maximum rate at a polysaccharide concentration of 6.7 mg/ml, Michaelis constant being 1.5 X 10(-6) M. Levanase remained stable at pH 5.4--7.5 and temperature 40--45 degrees C.
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