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  • Title: Embryonated eggs compared with fragments of chorioallantois attached to egg shell for isolation of Newcastle disease virus.
    Author: Brugh M, Erickson GA, Beard CW.
    Journal: Avian Dis; 1980; 24(2):486-92. PubMed ID: 7436966.
    Abstract:
    The feasibility of using fragments of chorioallantois attached to egg shell (C-S) as an alternative to embryonated eggs for isolating Newcastle disease virus (NDV) was assessed. In paired titrations of 6 strains of virus, embryonated eggs detected an average of 6-to-7-fold greater amounts of virus than did C-S fragments. The efficiency of isolation of viscerotropic velogenic NDV from 240 swab samples was 97% in embryonated eggs and 84% in C-S fragments. Sensitivity differences between the 2 host systems were revealed only in tests of samples expected to contain small amounts of virus. These results suggest that C-S fragments can be reliably used in some but not all instances, thereby relieving logistic constraints sometimes associated with the use of embryonated eggs.
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