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  • Title: Interlaboratory evaluation of four RIA kits for determination of plasma cortisol, with special reference to accuracy: influence of matrix in calibration standards.
    Author: Lantto O, Björkhem I, Blomstrand R, Kallner A.
    Journal: Clin Chem; 1980 Dec; 26(13):1899-902. PubMed ID: 7438443.
    Abstract:
    We used four RIA kits for determination of plasma cortisol to assay 12 plasma samples in four laboratories, and compared the results with those obtained with a highly accurate method based on isotope dilution/mass spectrometry. All kits (Farmos, NEN, Sorin, and Clinical Assays) showed 10-50% deviation from the comparison method in the normal range (200-700 nmol/L). The inter- and intralaboratory imprecision (CV) ranged between 8-27% and 5-11%, respectively. Possible explanations for this low accuracy are discussed. Retention of an intact biological matrix in the calibration standards seems to be one prerequisite for accuracy.
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