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  • Title: Triple-lumen perfusion of the canine jejunum.
    Author: Barbezat GO.
    Journal: Gastroenterology; 1980 Dec; 79(6):1243-5. PubMed ID: 7439632.
    Abstract:
    The triple-lumen gut perfusion technique was validated in five dogs with established jejunal fistulas. Transit time through the 30-cm test segment was 6.2 min when perfused at 4 ml/min with a balanced electrolyte solution (Na 140, K 5, Cl 110 and HCO3 35 mM), and this remained constant during a 3-hr test period. Fasting intestinal content was very small in the dog. After 35 min equilibration, steady-state absorption was achieved during perfusion with electrolyte solution containing polyethylene glycol (5 g/liter) as a nonabsorbable marker (10 studies). The triple-lumen gut perfusion technique is well suited to the study of intestinal transport in vivo in dogs with chronic intestinal fistulas.
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