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  • Title: Rubella-specific IgM detection using Sephacryl S-300 gel filtration.
    Author: Morgan-Capner P, Davies E, Pattison JR.
    Journal: J Clin Pathol; 1980 Nov; 33(11):1082-5. PubMed ID: 7440755.
    Abstract:
    Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2.5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.
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