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  • Title: 3-Methoxybenzidine: a potent inhibitor of cholesterol side chain cleavage cytochrome P-450.
    Author: Duval JF, Vickery LE.
    Journal: Steroids; 1980 Oct; 36(4):473-81. PubMed ID: 7444997.
    Abstract:
    The effect of 3-methoxybenzidine on the conversion of cholesterol to pregnenolone was investigated using a reconstituted enzyme system comprised of adrenodoxin, adrenodoxin reductase and cytochrome P-450scc purified from bovine adrenal cortex. Under conditions where the cytochrome P-450scc concentration was rate-limiting, 3-methoxybenzidine was found to be a potent inhibitor, causing 50% inhibition at 7 microM when using a cholesterol concentration of 70 microM. The parent compound, benzidine, was much less effective, exhibiting an I50 value of approximately 40 microM. No effect of 3-methoxybenzidine was observed on the adrenodoxin reductase and adrenodoxin-catalyzed reduction of cytochrome c by NADPH, and it is concluded that 3-methoxybenzidine acts on cytochrome P-450scc in inhibiting cholesterol side chain cleavage.
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