These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Rapid method of obtaining enriched fractions of rat brain neuronal and glial cells].
    Author: Shpakov AA, Kuznetsova VI, Savchenko ZI.
    Journal: Biull Eksp Biol Med; 1980 Nov; 90(11):630-2. PubMed ID: 7448404.
    Abstract:
    Cell suspension of the rat brain was separated into fractions of neuronal and glial cells (FNC, FGC) during 7-10 minutes. The suspension was filtered in the column through a series of filters with 400-600, 200 (FNC), 132 and 100 (FGC) mu pores. The purity of the fractions obtained was analyzed by light microscopy. According to a single cell counting the purity of FNC and FGC contituted 60 and 90%, respectively. The respective figures were 97 and 90% as shown by cell weight comparison. The biochemical and morphological findings indicate a good viability of isolated cells. It has been established that of total rat brain protein (108 mg per 1 g crude tissue), the proteins of nerve cells account for about 30%. The mass ratio of neurons to glial cells is 2.5:1. This fact should be taken into consideration during studies on the functional interactions between neurons and glial cells.
    [Abstract] [Full Text] [Related] [New Search]