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  • Title: Cytochemical properties of the conventional Schiff reagent fortified with Tris-buffer.
    Author: Dutt MK.
    Journal: Folia Histochem Cytochem (Krakow); 1980; 18(2):129-32. PubMed ID: 7450603.
    Abstract:
    This paper presents a cytochemical study of a modified Schiff reagent in which 250 Tris-buffer has been incorporated in addition to 5 ml of N HCL and 1.0 g of potassium metabisulphite per 100 ml of an aqueous solution of 0.5% basic fuchsin. The results indicate production of an orange-coloured dye-reagent which when used on acid hydrolysed tissue sections reveals brilliantly stained nuclei. The shelf-life of this modified Tris-Schiff reagent is also much more than that of the conventional Schiff reagent. The in vitro absorption spectra of the orange-coloured dye-reagent together with those of the modified Tris-Schiff reagent with formalin as well as the conventional Schiff reagent with formalin are also presented. Apossible explanation for the increased shelf-life has been offered.
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