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  • Title: Hexose transport and phosphorylation by Novikoff rat hepatoma cells as function of extracellular pH.
    Author: Wohlhueter RM, Plagemann PG.
    Journal: J Biol Chem; 1981 Jan 25; 256(2):869-75. PubMed ID: 7451478.
    Abstract:
    The effect of extracellular pH on hexose uptake by cultured Novikoff hepatoma cells has been examined with respect to four distinct parameters: i) the transport of 3-O-methyl-D-glucose, ii) the uptake of 2-deoxy-D-glucose by intact cells, iii) the phosphorylation of 2-deoxy-D-glucose in vitro by cell-free preparations, and iv) the intracellular pH as measured by 5,5'-dimethyl[2-14C]oxazolidine-2,4-dione. Transport per se was not affected by pH in the range of 6 to 8, while uptake of 2-deoxy-D-glucose increased with pH 2- to 3-fold over this range. The pH sensitivity of uptake can be explained on the basis of a change in hexokinase activity effective in situ. The pH dependence of hexokinase observed in vitro, however, was insufficient to account for that apparent in situ.
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