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  • Title: Light-enhanced cross-linking of rhodopsin in rod outer segment membranes as detected by chemical probes.
    Author: Shaw A, Crain R, Marinetti GV, O'Brien D, Tyminski PN.
    Journal: Biochim Biophys Acta; 1980 Dec 12; 603(2):313-21. PubMed ID: 7459357.
    Abstract:
    Bovine rod outer segment membranes were treated with cross-linking reagents before and after light exposure. Bleached membranes showed enhanced cross-linking with difluorodinitrobenzene or methyl acetimidate compared to dark-adapted membranes. The light-induced enhancement of cross-linking may be due to increased association of rhodopsin monomers in the light and/or due to increased reactivity of amino and sulfhydryl groups of bleached rhodopsin. In some instances, the band ascribed to the rhodopsin monomer in gel electrophoresis appears as a partially resolved doublet. Treatment of bleached rod outer segment membranes with methyl acetimidate improved the resolution of the doublet into two closely migrating bands.
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