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  • Title: Identification of a less immunoreactive form of prolactin in the rat pituitary.
    Author: Sinha YN, Gilligan TA.
    Journal: Endocrinology; 1981 Mar; 108(3):1091-4. PubMed ID: 7460835.
    Abstract:
    Disc electrophoretic analysis of prolactin (PRL) in the rat pituitary is usually performed using a 7.5% concentration of acrylamide gel. Re-electrophoresis of the eluate of the PRL band from such a gel on polyacrylamides of serially increasing concentrations revealed that the PRL band of 7.5% gel actually contains at least two additional proteins. One of these, whose apparent mol. wt. is 31,000, has significant pigeon crop-sac stimulating activity but very little or no cross-reactivity in a RIA for rat PRL. It also has a rapid turnover rate in short-term in vitro incubations. This newly-found protein seems to be a nonimmunoreactive molecular variant of rat PRL.
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