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  • Title: In vitro response of human peripheral blood lymphocytes of protein A. DNA synthesis and generation of cells synthesizing the three major classes of Ig.
    Author: Gausset P, Delespesse G, Duchateau J, Collet H.
    Journal: Immunology; 1980 Dec; 41(4):891-7. PubMed ID: 7461714.
    Abstract:
    Increased DNA synthesis and immunoglobulin secretion was observed in human peripheral blood lymphocytes (PBL) cultured in vitro with soluble protein A from Staphylococcus aureus (Sp-A). Optimal Ig secretion was obtained in 6 day cultures containing 1 x 10(6) cells/ml and 10 microgram/ml Sp-A. The presence of 5 x 10(-5) M 2-mercaptoethanol in the culture medium as well as careful selection of foetal calf serum were needed for optimal results. In response to Sp-A stimulation, the three main classes of immunoglobulins were secreted by PBL. In some individuals, concentration of Sp-A optimal for IgG and IgM secretion inhibited IgA production. Immunoglobulin-containing cells were less abundant (0.5--5% of total cells) and smaller in Sp-A than in PWM-stimulated cultures (5--15%). The data suggest that Sp-A and PWM stimulated different subsets of circulating lymphocytes.
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