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  • Title: Semi-conservative transcription of double-stranded RNA catalyzed by bacteriophage phi 6 RNA polymerase.
    Author: Emori Y, Iba H, Okada Y.
    Journal: J Biochem; 1980 Dec; 88(6):1569-75. PubMed ID: 7462197.
    Abstract:
    Treatment of Pseudomonas phaseolicola double-stranded RNA bacteriophage phi 6 with sodium deoxycholate converted the virions to nucleocapsids, which had in vitro RNA polymerase activity. The incorporation of [3H]UMP continued for at least 7 h. The initial incorporation was detected as intermediate RNA. Radioactivity was chased first into three segments of double-stranded RNA, and then into small, medium, and large species of single-stranded RNA successively via the intermediate RNA. Several copies of single-stranded RNA at least were synthesized from a template. The RNA synthesis clearly took place by a semi-conservative mechanism with respect to templates. That is, 5-bromo UTP was incorporated into one strand of double-stranded RNA to make a hybrid RNA of brominated and unbrominated strands. Furthermore, one strand of the 3H-labeled parental double-stranded RNA was shown to be released as single-stranded RNA.
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