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  • Title: In vitro development of CFU-E and BFU-E in cultures of embryonic and post-embryonic chicken hematopoietic cells.
    Author: Samarut J, Bouabdelli M.
    Journal: J Cell Physiol; 1980 Dec; 105(3):553-63. PubMed ID: 7462339.
    Abstract:
    A culture method is proposed for the in vitro development of chicken erythrocytic progenitors. When grown with avian erythropoietin, Colony Forming Unit Erythrocytic (CFU-E) and Burst Forming Unit-Erythrocytic (BFU-E) give rise respectively to erythrocytic colonies and bursts within 3 and 6 days. BFU-E development is greatly enhanced by pokeweed-mitogen-spleen-cell conditioned medium and requires higher erythropoietin concentrations than for CFU-E. An antigen specific to immature red cells can be detected on CFU-E but not on BFU-E, showing that both progenitors represent distinct entities. BFU-E and CFU-E are found in embryonic marrow and yolk sac. In the young blastoderm BFU-E becomes detectable at the primitive streak stage.
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