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  • Title: Glutathione-prostaglandin A1 conjugate as substrate in the purification of prostaglandin 9-ketoreductase from rabbit kidney.
    Author: Toft BS, Hansen HS.
    Journal: Prostaglandins; 1980 Oct; 20(4):735-46. PubMed ID: 7465864.
    Abstract:
    Using GSH-PGA1 as substrate for determination of enzyme activity a pI 4.8 form of rabbit kidney prostaglandin 9-keto-reductase has been purified 95 times to a specific activity of 1755 nmol/min per mg protein. The purification procedures involve ion-exchange chromatography, gel-filtration and affinity chromatography. The latter procedure comprises Blue Sepharose affinity chromatography, and GSH-PGA1-Sepharose affinity chromatography. The purified enzyme preparation also showed a weak NADP+-dependent 15-hydroxyprostaglandin dehydrogenase activity, 20 nmol/min per mg protein with PGE1 as substrate. Km (PGE1) for the dehydrogenase is 142.6 +/- 45.1 microM (S.E., n = 7).
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