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  • Title: Permeability of lactating-rat mammary gland Golgi membranes to monosaccharides.
    Author: White MD, Kuhn NJ, Ward S.
    Journal: Biochem J; 1980 Sep 15; 190(3):621-4. PubMed ID: 7470073.
    Abstract:
    The Golgi-membrane vesicles present in particulate preparations of lactating rat mammary gland were biosynthetically loaded with [14C]lactose. This lactose was effectively retained by particles sedimented after exposure to 0.25 M-disaccharide, but was partly lost after exposure to 0.25 M-glucose or other solutes of similar size. Loss of lactose was time-, concentration- and temperature-dependent and varied with the solute structure. This behaviour is ascribed to the presence of protein in the Golgi membrane, forming a specific carrier or channel that serves to supply glucose for lactose synthesis.
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