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Title: Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts. Author: Jones AV, Harwood JL. Journal: Biochem J; 1980 Sep 15; 190(3):851-4. PubMed ID: 7470086. Abstract: When [14C]diacylgalactosylglycerol was added to isolated pea or lettuce chloroplasts linolenate synthesis was seen. The desaturation of [14C]linoleate in diacylgalactosylglycerol to [14C]linolenate was stimulated by the addition of a soluble protein fraction containing lipid-exchange activity. Other [14C]acyl lipids were ineffective, except that [14C]phosphatidylcholine in the presence of UDP-galactose and sn-glycerol 3-phosphate could also supply [14C]linoleate for desaturation. These results are consistent with a role of diacylgalactosylglycerol in linolenate synthesis, as indirectly suggested by labelling experiments.[Abstract] [Full Text] [Related] [New Search]