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  • Title: Separation of collagen cyanogen bromide-derived peptides by reversed-phase high-performance liquid chromatography.
    Author: van der Rest M, Bennett HP, Solomon S, Glorieux FH.
    Journal: Biochem J; 1980 Oct 01; 191(1):253-6. PubMed ID: 7470094.
    Abstract:
    A new technique for separation of the CNBr cleavage products of collagen is described. It involves the use of a 30 nm pore reversed-phase high-performance liquid chromatography column eluted with a linear gradient of acetonitrile/water containing 0.01 M-heptafluorobutyric acid. The separation of type I, type II and type III CNBr peptides is described. Resolution is particularly good for the low-molecular-weight peptides. The method is fast, quantitative and sensitive, and the complete volatility of the eluent facilitates the recovery of the separated peptides.
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