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Title: Midpoint potentials of cytochromes in vesicles of anaerobically-grown Paracoccus denitrificans determined by the indirect coulometric titration method. Author: Kula T, Stellwagen E, Szentirmay R, Kuwana T. Journal: Biochim Biophys Acta; 1981 Feb 12; 634(2):279-88. PubMed ID: 7470501. Abstract: 1. Multiplicity of redox components with spectral properties similar to b-type cytochromes was established in vesicles derived fro anaerobically-grown Paracoccus denitrificans. 2. Multiplicity of c-type cytochromes was not apparent either from low temperature spectroscopy or potentiometric titrations. 3. Cytochromes a + a3 and a component, only observable at liquid nitrogen temperature, with a spectral maximum at 582.5 nm were detected. 4. Redox cycling of electron transport components using the indirect coulometric titration method was a convenient means of pairing redox potentials and was reproducible in total absorbance changes, midpoint potentials and spectral maxima.[Abstract] [Full Text] [Related] [New Search]