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  • Title: Liquid-chromatographic analysis for urinary porphyrins.
    Author: Ford RE, Ou CN, Ellefson RD.
    Journal: Clin Chem; 1981 Mar; 27(3):397-401. PubMed ID: 7471387.
    Abstract:
    Urinary porphyrins are separated, according to number of carboxyl groups, in a system consisting of a mu-Bondapak C18 stationary phase and a mobile phase of methanol and aqueous sodium phosphate (pH 3.5) in a linear gradient. The specimen is prepared simply by adjusting the pH of a 5-mL sample to 2.0 and removing solids by centrifugation. The eluted porphyrins are measured fluorometrically. Naturally occurring non-porphyrin fluorescent substances are eluted ahead of the porphyrins. Chromatography requires about 20 min, and a re-establishment of initial conditions requires an additional 15 min.
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