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  • Title: [Analysis of 17-oxosteroids by gas liquid chromatography after purification of the urinary extract by Girard's T reagent (author's transl)].
    Author: Habrioux G.
    Journal: Ann Biol Clin (Paris); 1978; 36(5):433-42. PubMed ID: 747250.
    Abstract:
    The authors describe a method of fractionation of urinary 17-oxosteroids after purification of the urinary extract by Girard's T reagent. After solvolysis, enzyme hydrolysis and purification by Girard's T reagent, the 17-oxosteroids were estimated in the form of trimethyl silyl ethers by gas liquid chromatographye on OV 225 using cholesterol acetate for standardisation. The improvement in the quality of the fraction using this technic, the analytical characteristics of which are studied in this work, is of definite interest, in particular after stimulation by ACTH, HCG, metopirone and in the case of pigmentation of the urine.
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