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  • Title: [The plasma concentration measurement of local anesthetics, thiobarbiturates and mexiletine by HPLC with automatic pre-treatment system].
    Author: Kakinohana O, Okuda Y.
    Journal: Masui; 1995 Aug; 44(8):1165-70. PubMed ID: 7474321.
    Abstract:
    The concentrations of lidocaine, its metabolites (MEGX, GX), mepivacaine, bupivacaine, thiamylal, thiopental and mexiletine in plasma have been determined by HPLC method with automatic pre-treatment system. The drugs carried by pre-treatment mobile phase are trapped by hydrophobic interaction on the pre-treatment column (4 mm i.d. x 30 mm L., tetramethylolmethanetriacrylate resin packing). As sending analytical mobile phase which was conditioned with 0.1 M sodium phosphate buffer (pH 2.1) and acetonitrile by switching the flow changeover valve, the absorped drugs were eluted quickly and introduced to the analytical column (6 mm i.d x 150 mm L., octadecylsilan chemical bond sillica packing). On this column, the drugs were separated and analyzed, then measured by a UV spectrophotmetric detector. The time of switching valve, the wave length of detector for measurement and the buffer/acetonitrile ratio of analytical mobile phase were specific for the drugs, respectively. And high recovery and reproducibility were obtained for using auto-injector, so it has been possible to determine the concentrations of drugs in plasma with absolute calibration curves.
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