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  • Title: [Characteristics of the RFLP marker of the D13S25 locus on human chromosome 13: mapping of SspI sites in the pH2-42 probe].
    Author: Aitova SS, Sulimova GE, Udina IG.
    Journal: Mol Biol (Mosk); 1995; 29(4):813-6. PubMed ID: 7476947.
    Abstract:
    Analysis of probe pH2-42 of locus D13S25 in genomic DNA in Moscow population has been made by PCR-RFLP using restriction endonuclease SspI. Localization of additional SspI-site at position 1240 (which has been revealed earlier by sequencing of pH2-42) was confirmed. This SspI restriction site was present in all representatives of the population, thus being not polymorphic. Consequently establishment of identity of additional site at position 1240 with polymorphic SspI site in D13S25 studied in previous investigations, requires additional data from another populations. Possible localization of polymorphic SspI-site in locus D13S25 is discussed.
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