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  • Title: Comparison of histologic stains in the diagnosis of Pneumocystis carinii.
    Author: Naimey GL, Wuerker RB.
    Journal: Acta Cytol; 1995; 39(6):1124-7. PubMed ID: 7483986.
    Abstract:
    OBJECTIVE: To evaluate commonly used histologic stains used to detect Pneumocystis, including Diff-Quik (DQ), Grocott's Gomori methenamine silver (GMS), Papanicolaou, and hematoxylin and eosin (H&E). STUDY DESIGN: The study compared the ability of these stains to detect alveolar casts. RESULTS: Although there was a slightly higher average number of casts detected with DQ, the difference between this stain and GMS was not significant. Both Papanicolaou stain and H&E showed significantly lower averages. Some differences in H&E may be related to differences in preparation. CONCLUSION: Similar results were obtained with DQ and GMS, but DQ offered the advantage of ease of preparation, rapidity and cost-effectiveness.
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