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  • Title: [Cloning and expression of the Escherichia coli thioredoxin gene].
    Author: Barenboĭm MG, Shingarova LN, Korobko VG.
    Journal: Bioorg Khim; 1995 Jul; 21(7):524-7. PubMed ID: 7488267.
    Abstract:
    The gene encoding thioredoxin (trxA) was isolated from chromosomal DNA of E. coli HB101 strain using the polymerase chain reaction. The cloned structural gene with a synthetic Shine-Dalgarno sequence was placed under the control of either inducible tac-promoter or a tandem of two strong constitutive promoters A2 and A3 from early region of bacteriophage T7. Both constructions were shown to provide high levels of biosynthesis of recombinant thioredoxin.
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