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  • Title: Human GLUT-2 overexpression does not affect glucose-stimulated insulin secretion in MIN6 cells.
    Author: Ishihara H, Asano T, Tsukuda K, Katagiri H, Inukai K, Anai M, Yazaki Y, Miyazaki J, Kikuchi M, Oka Y.
    Journal: Am J Physiol; 1995 Nov; 269(5 Pt 1):E897-902. PubMed ID: 7491941.
    Abstract:
    Accumulated evidence suggests that GLUT-2, in addition to its role in glucose transport, may also have other functions in glucose-stimulated insulin secretion. As a first step in addressing this possibility, we have engineered MIN6 cells overexpressing human GLUT-2 by transfection with human GLUT-2 cDNA. Stable transformants harboring human GLUT-2 cDNA exhibited an approximately twofold increase in 3-O-methyl-D-glucose uptake at 0.5 and 15 mM. Glucokinase activity or glucose utilization measured by conversion of [5-3H]glucose to [3H]H2O was not, however, altered in the MIN6 cells overexpressing human GLUT-2. Furthermore, glucose-stimulated insulin secretion was not affected by over-expression of human GLUT-2. An abundance of GLUT-2, therefore, does not correlate with the glucose responsiveness of cells in which glycolysis is regulated at the glucose phosphorylating step. These data suggest that GLUT-2 by itself does not have significant functions other than its role in glucose transport in glucose sensing by MIN6 cells.
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