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  • Title: Determination of sulphonamides in human urine by azo dye precolumn derivatization and micellar liquid chromatography.
    Author: Simó-Alfonso EF, Ramis-Ramos G, García-Alvarez-Coque MC, Esteve-Romero JS.
    Journal: J Chromatogr B Biomed Appl; 1995 Aug 04; 670(1):183-7. PubMed ID: 7493079.
    Abstract:
    A high-performance liquid chromatographic method for the determination of sulphonamides in urine is reported. The drugs (sulphadiazine, sulphaguanidine, sulphamethizole, sulphamethoxazole, and sulphathiazole) were diazotized with nitrite and coupled with N-(1-naphthyl)ethylenediamine dihydrochloride in a sodium dodecyl sulphate (SDS) micellar medium. Separation of the sulphonamide azo dyes was performed on a C18 column with a 0.05 M SDS-2.4% pentanol mobile phase, which permitted the direct injection of the urine samples. The limits of detection were in the 0.1-0.3 micrograms/ml range.
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