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  • Title: The beta subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase.
    Author: Severinov K, Mustaev A, Severinova E, Kozlov M, Darst SA, Goldfarb A.
    Journal: J Biol Chem; 1995 Dec 08; 270(49):29428-32. PubMed ID: 7493980.
    Abstract:
    Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase-promoter complex were cross-linked to the beta subunit. Using limited proteolysis and chemical degradation, the cross-link was mapped to a segment of beta between amino acids Val516 and Arg540. This region (Rif-cluster I) is known to harbor many rifampicin-resistant (RifR) mutations. The results demonstrate that Rif-culster I is part of the "5'-face" of the active center and provide structural basis for the long-known effects of RifR mutations on transcription initiation, elongation, and termination.
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