These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels.
    Author: Swain M, Ross NW.
    Journal: Electrophoresis; 1995 Jun; 16(6):948-51. PubMed ID: 7498141.
    Abstract:
    A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.
    [Abstract] [Full Text] [Related] [New Search]