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  • Title: Is Kaposi's-sarcoma-associated herpesvirus detectable in semen of HIV-infected homosexual men?
    Author: Lin JC, Lin SC, Mar EC, Pellett PE, Stamey FR, Stewart JA, Spira TJ.
    Journal: Lancet; 1995 Dec 16; 346(8990):1601-2. PubMed ID: 7500753.
    Abstract:
    We explored a possible route of transmission of Kaposi's-sarcoma-associated herpes virus (KSHV) with nested and unnested PCR techniques. We looked for KSHV DNA sequences in semen of HIV-positive homosexual men and HIV-negative healthy semen donors. With unnested primers we found KSHV sequences in 21 of 33 (64%) homosexual men and in none of 30 healthy donors. With a nested PCR assay, 30 of 33 (91%) specimens from the homosexual men and 7 of 30 (23%) specimens from healthy donors had detectable KSHV sequences. Over 5 years of follow-up, 13 of 30 KSHV-positive homosexual men (43%) developed KS compared with none of the 3 KSHV-negative homosexual men.
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