These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Na+ dependent Ca2+ influx induced by depolarization in neurons dissociated from rat nucleus basalis.
    Author: Tatsumi H, Katayama Y.
    Journal: Neurosci Lett; 1995 Aug 18; 196(1-2):9-12. PubMed ID: 7501266.
    Abstract:
    Neurons were acutely dissociated from the rat nucleus basalis, and whole-cell patch clamp recordings were made. Voltage dependent calcium currents (ICa) were recorded and fura-2 microfluorimetric recordings of intracellular free Ca2+ concentration ([Ca2+]i) were made at the same time. In Na(+)-containing solution, a depolarization from -60 to +40 mV evoked the maximal increase in [Ca2+]i, and this decreased to 43% of the maximal with a large depolarization to +120 mV. The [Ca2+]i increase induced by the large depolarization (+20 to +120 mV) was inhibited by perfusion of Na(+)-free external solution, and was less when the recording pipette contained a peptide (PRLLFYKYVYKRYRAGKQRG, named XIP) known to inhibit Na/Ca exchange. These results suggest that the [Ca2+]i increase by the large depolarization is mediated by reverse operation of Na/Ca exchange (Ca2+ inward and Na+ outward).
    [Abstract] [Full Text] [Related] [New Search]