These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Comparison of human myofibrillar protein catabolic rate derived from 3-methylhistidine excretion with synthetic rate from muscle biopsies during L-[alpha-15N]lysine infusion.
    Author: McKeran RO, Halliday D, Purkiss P.
    Journal: Clin Sci Mol Med; 1978 May; 54(5):471-5. PubMed ID: 750147.
    Abstract:
    1. Urine was collected in five healthy men over 10--14 days, with fasting blood samples on days 1, 5 and 10, whilst they consumed a standard creatine-free diet, which was quantitatively related to their body surface area. 2. The urinary excretion of 3-methylhistidine fell to a plateau by day 5 in all subjects. Myofibrillar protein catabolic rate calculated from the mean value of 3-methylhistidine excretion from day 5 to day 10 averaged 1.21 g day-1 kg-1 body weight. The average turnover of muscle myofibrillar protein was calculated to be 2.16%/day. 3. From a previous study using continuous intravenous infusion of L-[alpha-15N]lysine with serial muscle biopsies on the same subjects, the mean myofibrillar protein synthetic rate was calculated to be 0.82 g day-1 kg-1 body weight, and the mean turnover rate was 1.47%/day of total muscle myofibrillar protein. 4. The estimations of myofibrillar protein turnover rate derived from the two methods are compared and the differences discussed.
    [Abstract] [Full Text] [Related] [New Search]